CBX4 Rabbit Polyclonal Antibody

SKU: orb182925

Description

CBX4 Rabbit Polyclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ICC, WB
• Dilution Range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine, Equine, Porcine, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CBX4 (401-500/560aa)
• Target: CBX4
• Molecular Weight: 61 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NBP16; PC2; MPc2; CBX4_HUMAN; CBX4; Chromobox protein homolog 4; Polycomb 2 homolog (Pc2 | hPc2); 2.3.2.-; CBX4_MOUSE; E3 SUMO-protein transferase CBX4; Polycomb 2 homolog (Pc2 | mPc2);

Hela cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CBX4) polyclonal Antibody, Unconjugated (orb182925) 1:25, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Lane 1: Mouse Liver tissue lysates, Lane 2: Rat Liver tissue lysates, Lane 3: Human K562 cell lysates, Lane 4: Human HL-60 cell lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human HepG2 cell lysates, Lane 7: Human HUVEC cell lysates, Lane 8: Human A549 cell lysates, Primary: Anti-CBX4 (orb182925) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 61 kDa, Observed band size: 61 kDa.

Quick Database Links

Gene Symbol

CBX4

UniProt

O00257