CBX3 antibody

• Catalog Number: orb19108
• Category: Antibodies
• Description: Goat polyclonal antibody to CBX3
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:32000, WB: 0.3-1.0 μg/ml, IHC-P: 2-3μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
• Conjugation: Unconjugated
• MW: 20.8
• Target: CBX3 / HP1 Gamma
• Entrez: 11335
• Protein Sequence: EAFLNSQKAGKEKD
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CBX3 antibody, anti HP1-GAMMA antibody, anti
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded Human Placenta shows strong nuclear staining in trophoblasts. Recommended concentration, 2-3ug/ml.WB: Approx. 20kDa band observed in lysates of A431, HeLa and HepG2. (calculated MW of 20.8kDa according to NP_009207.2 and NP_057671.2). Recommended concentration: 0.3-1.0 μg/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of NIH3T3 nuclear cell lysate using CBX3 antibody.

Immunohistochemical staining of Human Placenta using CBX3 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using CBX3 antibody.

Immunofluorescence analysis of paraformaldehyde-fixed U2OS cells using CBX3 antibody.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using CBX3 antibody.

Western blot analysis of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and K562 (E) nuclear cell lysate using CBX3 antibody.