HP1 gamma/CBX3 Antibody

• Catalog Number: orb402234
• Category: Antibodies
• Description: HP1 gamma/CBX3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human HP1 gamma recombinant protein (Position: A2-Q183).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, Rat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 21 kDa
• UniProt ID: Q13185
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Chromobox protein homolog 3; HECH; Heterochromatin
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-HP1 gamma antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-HP1 gamma antibody.Lane 1:rat brain tissue; 2:rat spleen Tissue; 3:rat testis Tissue; 4:rat PC-12 Cell; 5:mouse brain Tissue; 6:mouse brain Tissue; 7:mouse testis Tissue; 8:mouse HEPA1-6 Cell; 9:mouse NIH/3T3 Cell.

WB analysis of HP1 gamma using anti-HP1 gamma antibody.Lane 1:human HeLa Cell; 2:human MCF-7 Cell; 3:human COLO320 Cell; 4:human HepG2 Cell; 5:human placenta Tissue; 6:human A549 Cell; 7:human SKOV-3 Cell; 8:human PANC-1 Cell.

IF analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in immunocytochemical section of U20S cell.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in paraffin-embedded section of human mammary cancer tissue.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in paraffin-embedded section of mouse small intestine tissue.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in paraffin-embedded section of rat small intestine tissue.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in paraffin-embedded section of human rectal cancer tissue.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in frozen section of rat spleen tissues.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in frozen section of human placenta tissues.

IHC analysis of HP1 gamma using anti-HP1 gamma antibody.HP1 gamma was detected in frozen section of mouse spleen tissues.