CBL monoclonal antibody (M01), clone 6D12

• Catalog Number: orb2295021
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant CBL.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 6D12
• Tested applications: ELISA, IP, WB
• Reactivity: Human
• Isotype: IgG1 Kappa
• Immunogen: CBL (NP_005179, 736 a.a. ~ 835 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: NIQSQAPSITESSTFGEGNLAAAHANTGPEESENEDDGYDVPKPPVPAVLARRTLSDISNASSSFGWLSLDGDPTTNVTEGSQVPERPPKPFPRRINSER
• NCBI: NP_005179
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

CBL monoclonal antibody (M01), clone 6D12 Western Blot analysis of CBL expression in K-562.

Detection limit for recombinant GST tagged CBL is 0.1 ng/ml as a capture antibody.

Immunoprecipitation of CBL transfected lysate using anti-CBL monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CBL monoclonal antibody.

Western Blot analysis of CBL expression in transfected 293T cell line by CBL monoclonal antibody (M01), clone 6D12. Lane 1: CBL transfected lysate (Predicted MW: 99.6 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (36.74 KDa).