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Catalog Number | orb1524623 |
---|---|
Category | Antibodies |
Description | CBF-beta (isoform 2) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IP, WB |
Reactivity | Human |
Immunogen | between 166 and 182 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:1,000 - 1:5,000; IP - 2 - 10 µg/mg lysate |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CBF-beta (isoform 2) |
UniProt ID | Q13951 |
NCBI | NP_001746.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | polyomavirus enhancer binding protein 2, beta subu Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of Isoform 2 of human CBF-beta by western blot and immunoprecipitation. Samples: Whole cell lysate from Jurkat (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) and HeLa (H; 50 µg) cells. Antibodies: Affinity purified rabbit anti-CBF-beta (isoform 2) antibody orb1524623 used for WB at 0.4 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. Isoform 2 of CBF-beta was also immunoprecipitated by rabbit anti-CBF-beta antibodies, which recognize upstream epitopes that are common to isoform 1 and isoform 2. Isoform 2 of CBF-beta was not immunoprecipitated by rabbit anti-CBF-beta (isoform 1) antibody orb1524621. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
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