CBF beta Antibody / CBFB

• Catalog Number: orb2634803
• Category: Antibodies
• Description: The transcription factor polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (osteoblast-specific transcription factor), CBFB (core binding factor subunit beta) and AML3 (acute myeloid leukemia), is composed of two subunits and is essential for the regulation of hematopoiesis and osteogenesis.
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2a
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: Recombinant full-length human protein was used as the immunogen for the CBF beta antibody.
• UniProt ID: Q13951
• Tested applications: ELISA, FACS, IF, WB
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml
• Application notes: Optimal dilution of the CBF beta antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-CBFB-1E6
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This CBF beta antibody is available for research use only.
• Note: For research use only

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CBF beta antibody (clone PCRP-CBFB-1E6). These results demonstrate the foremost specificity of the PCRP-CBFB-1E6 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

FACS staining of PFA-fixed human HeLa cells with CBF beta antibody (blue, clone PCRP-CBFB-1E6) and isotype control (red).

Immunofluorescent staining of PFA-fixed human human U-87 cells using CBF beta antibody (green, clone PCRP-CBFB-1E6) and phalloidin (red).

Western blot testing of human HeLa cell lysate using CBF beta antibody (clone PCRP-CBFB-1E6). Predicted molecular weight ~22 kDa.

SDS-PAGE analysis of purified, BSA-free CBF beta antibody (clone PCRP-CBFB-1E6) as confirmation of integrity and purity.