CAVIN1 Rabbit Polyclonal Antibody

SKU: orb576159

Description

Rabbit polyclonal antibody to CAVIN1

Research Area

Cell Biology

Images & Validation

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Item 1 of 6

Tested Applications	IHC, WB
Reactivity	Human, Mouse
Predicted Reactivity	Bovine, Canine, Equine, Rabbit, Rat

Related Conjugates & Formulations

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Biotin

FITC

HRP

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Immunogen	The immunogen is a synthetic peptide directed towards the middle region of mouse PTRF
Target	CAVIN1
Protein Sequence	Synthetic peptide located within the following region: SEALPEKEGDELGEGERPEDDTAAIELSSDEAVEVEEVIEESRAERIKRS
Molecular Weight	43 kDa
Purification	Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and may contain up to 2% sucrose.
Concentration	0.5 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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C, Pt, cav, Ptrf, Cavin, Cav-p60, AW546441, 2310075E07Rik

Quality Guarantee

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25 ug of the indicated Mouse tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Sample Tissue: Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.

Sample Tissue: Mouse Lung, Antibody Dilution: 1.0 ug/ml.

IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.

Lanes: Lane 1: 50 ug mouse embryonic fibroblast lysate, Lane 2: 50 ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50 ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein, Primary Antibody Dilution: 1:1000, Secondary Antibody: Donkey anti-Rabbit-HRP, Secondary Antibody Dilution: 1:10000, Gene Name: PTRF.

WB Suggested Anti-PTRF Antibody Titration: 0.625 ug/ml, ELISA Titer: 1:312500, Positive Control: NIH/3T3 cell lysate.

Quick Database Links

Gene Symbol

CAVIN1

UniProt

O54724

RefSeq (Protein):NP_033012

UniProt Details

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No UniProt data available

NCBI Reference Sequences

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Associated Accession Numbers

Curated reference sequences for the gene transcript and protein product

Protein	NP_033012

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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