CAVIN1 Rabbit Polyclonal Antibody

• Catalog Number: orb576159
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CAVIN1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Rabbit, Rat
• Reactivity: Human, Mouse
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of mouse PTRF
• Concentration: 0.5 mg/ml
• Form/Appearance: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and may contain up to 2% sucrose.
• Conjugation: Unconjugated
• MW: 43 kDa
• Target: CAVIN1
• UniProt ID: O54724
• Protein Sequence: Synthetic peptide located within the following region: SEALPEKEGDELGEGERPEDDTAAIELSSDEAVEVEEVIEESRAERIKRS
• NCBI: NP_033012
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and may contain up to 2% sucrose.
• Alternative names: C, Pt, cav, Ptrf, Cavin, Cav-p60, AW546441, 231007
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Mouse tissue extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/ml of the antibody was used in this experiment.

Sample Tissue: Human Fetal Lung, Antibody Dilution: 1.0 ug/ml.

Sample Tissue: Mouse Lung, Antibody Dilution: 1.0 ug/ml.

IHC Information: Paraffin embedded mouse vessel and adipocytes (skeletal muscle) tissue, tested with an antibody Dilution of 5 ug/ml.

Lanes: Lane 1: 50 ug mouse embryonic fibroblast lysate, Lane 2: 50 ug mouse PTRF-KO embryonic fibroblast lysate, Lane 3: 50 ug mouse PTRF-KO embryonic fibroblast lysate+FLAG-PTRF protein, Primary Antibody Dilution: 1:1000, Secondary Antibody: Donkey anti-Rabbit-HRP, Secondary Antibody Dilution: 1:10000, Gene Name: PTRF.

WB Suggested Anti-PTRF Antibody Titration: 0.625 ug/ml, ELISA Titer: 1:312500, Positive Control: NIH/3T3 cell lysate.