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Catalog Number | orb19043 |
---|---|
Category | Antibodies |
Description | Goat polyclonal antibody to Caveolin 1 |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF, IHC, WB |
Reactivity | Canine, Human, Mouse, Porcine, Rat |
Dilution range | ELISA: 1:8000, WB: 0.001-0.01 μg/ml, IHC-P: 2.5μg/ml, FACS: 10ug/ml, IF/ICC: 10μg/ml. |
Conjugation | Unconjugated |
MW | 20.5; 17.0 |
Target | Caveolin 1 |
Entrez | 857 |
Protein Sequence | DELSEKQVYDAH |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | anti BSCL3 antibody; anti CAV 1 antibody; anti CAV Read more... |
Note | For research use only |
Application notes | ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 21kDa and 25kDa observed in Human Heart, Lung and Spleen lysates. These bands correspond to earlier findings in literature with different antibodies (Wiechen et al Am J Pathol. 2001 Mar;158(3):833-9, PMID: 11238032 ). In transfected HEK293 transiently expressing Caveolin 1 a band of approx. 23kDa is observed. This band is not observed in the non-transfected HEK293. This protein has a calculated MW of 20.5kDa according to NP_001744.2. Recommended concentration: 0.3-1 μg/ml. |
Expiration Date | 12 months from date of receipt. |
WB analysis of A431 (A) and A549 cell lysate using Caveolin 1 antibody
WB analysis of Human Heart using Caveolin 1 antibody
Immunofluorescence analysis of A431 cells using Caveolin 1 antibody
Immunofluorescence analysis of U2OS cells using Caveolin 1 antibody
Flow cytometric analysis of HeLa cells using Caveolin 1 antibody
Immunohistochemical staining of Human Uterus using Caveolin 1 antibody.
IF, IHC, IHC-Fr, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
IHC-P, WB | |
Human, Mouse, Porcine, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IF, IHC, IHC-Fr, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, FC, IF, WB | |
Canine, Mouse, Porcine, Rat | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
IHC-P, WB | |
Bovine, Canine, Equine, Porcine, Rabbit, Sheep | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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