Caveolin-1/CAV1 Antibody

• Catalog Number: orb215919
• Category: Antibodies
• Description: Caveolin-1/CAV1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC, IHC-Fr, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Caveolin-1 recombinant protein (Position: G4-I178). Human Caveolin-1 shares 95% and 94% amino acid (aa) sequences identity with mouse and rat Caveolin-1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Mouse, - Immunocytochemistry , 0.5-1μg/ml, Human, - Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 20472 MW
• UniProt ID: Q03135
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caveolin-1;CAV1;CAV;
• Note: For research use only
• Application notes: WB: The detection limit for Caveolin-1 is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-Caveolin-1/CAV1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis using anti-Caveolin-1/CAV1 antibody.Lane 1:HeLa cell;2:A549 cell;3:human placenta tissue;4:human A431 cell;5:human HL-60 cell;6:rat ovary tissue;7:rat heart tissue;8:mouse ovary tissue;9:mouse heart tissue.

IF analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody. Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue.

IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody. Caveolin-1/CAV1 was detected in a paraffin-embedded section of human glioma tissue.

IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody. Caveolin-1/CAV1 was detected in a paraffin-embedded section of human meningeoma tissue.

IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody. Caveolin-1/CAV1 was detected in a paraffin-embedded section of mouse brain tissue.

IHC analysis of Caveolin-1/CAV1 using anti-Caveolin-1/CAV1 antibody. Caveolin-1/CAV1 was detected in a frozen section of human placenta tissue.