Cav3.1 Antibody: APC

• Catalog Number: orb149911
• Category: Antibodies
• Description: Mouse monoclonal antibody against CaV3.1 conjugated to APC.
• Target: Cav3.1
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgG1
• Conjugation: APC
• Reactivity: Human, Mouse, Rat
• Concentration: 1 mg/ml
• Buffer/Preservatives: 95.64mM Phosphate, 2.48mM MES and 2mM EDTA
• Purification: Protein G Purified
• Immunogen: Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1
• UniProt ID: Q9WUT2
• MW: 200kDa
• Tested applications: ICC, IF, IHC
• Dilution range: WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
• Application notes: 1 µg/ml of SMC-405 was sufficient for detection of Cav3.1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
• Clone Number: N178A/9 (Formerly sold as S178A-9)
• Storage: Conjugated antibodies should be stored according to the product label
• Alternative names: CACNA1G antibody, CaV T1 antibody, Cav3 1 antibody
• Research Area: Neuroscience, Pharmacology & Drug Discovery
• Note: For research use only
• Entrez: 12291
• NCBI: NP_001106284.1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.1 Monoclonal Antibody, Clone N178A/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.1 Monoclonal Antibody, Clone N178A/9. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav3.1 Antibody. (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of Cav3.1 Calcium Channel protein using Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody, Clone N178A/9. Primary Antibody: Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody at 1:1000.