Cav3.1 Antibody (APC)

SKU: orb149911

Description

Mouse monoclonal antibody against CaV3.1 conjugated to APC.

Research Area

Neuroscience, Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ICC, IF, IHC, WB
• Dilution range: WB (1:1000), ICC/IF (1:100);optimal dilutions for assays should be determined by the user.
• Reactivity: Human, Mouse, Rat
• Application Notes:
  1 µg/ml of SMC-405 was sufficient for detection of Cav3.1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone No.: N178A/9 (Formerly sold as S178A-9)
• Immunogen: Fusion protein amino acids 2052-2172 (cytoplasmic C-terminus) of mouse Cav3.1
• Target: Cav3.1
• Molecular Weight: 200kDa
• Purification: Protein G Purified
• Conjugation: APC

Storage & Handling

• Storage: Conjugated antibodies should be stored according to the product label
• Buffer/Preservatives: 95.46mM Phosphate, 2.48mM MES and 2mM EDTA
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Voltage-dependent T-type calcium channel subunit alpha-1G, Cav3.1, CACNA1G, CAC1G_HUMAN, KIAA1123, CaV T1, Cav3 1, cav3 1c, MGC117234, NBR13, voltage gated calcium channel cav3.1, calcium channel voltage dependent alpha 1G subunit, calcium channel voltage dependent T type alpha 1G subunit, calcium channel voltage dependent T type alpha1G subunit, voltage dependent calcium channel alpha 1G subunit isoform 11, voltage dependent T type calcium channel subunit alpha 1G, Voltage gated calcium channel subunit alpha Cav3 1

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.1 Monoclonal Antibody, Clone N178A/9. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:50 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Cav3.1 Antibody (D) Composite.

Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Cav3.1 Monoclonal Antibody, Clone N178A/9. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Cav3.1 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell Membrane, Membrane, Cytoplasm, Nucleoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Cav3.1 Antibody. (D) Composite.

Western Blot analysis of Rat brain membrane lysate showing detection of Cav3.1 Calcium Channel protein using Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody, Clone N178A/9. Primary Antibody: Mouse Anti-Cav3.1 Calcium Channel Monoclonal Antibody at 1:1000.

Quick Database Links

Gene Symbol

Cav3.1

UniProt

Q9WUT2

NCBI Gene ID

12291

RefSeq (Protein):NP_001106284.1