Cathepsin D/CTSD Antibody

• Catalog Number: orb334587
• Category: Antibodies
• Description: Cathepsin D/CTSD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Cathepsin D recombinant protein (Position: M201-L412). Human Cathepsin D shares 84.4% and 84% amino acid (aa) sequence identity with mouse and rat Cathepsin D, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 44552 MW
• UniProt ID: P07339
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cathepsin D;3.4.23.5;Cathepsin D light chain;Cathe
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of SiHa cells using anti-CTSD antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Cathepsin D using anti-Cathepsin D antibody.Lane 1:HEPG2 cell;2:A549 cell;3:PANC1 cell.

IF analysis of CTSD using anti-CTSD antibody. CTSD was detected in immunocytochemical section of MCF-7 cells.

IHC analysis of Cathepsin D using anti-Cathepsin D antibody.Cathepsin D was detected in paraffin-embedded section of human intestinal cancer tissues.