Cathepsin D/CTSD Antibody

• Catalog Number: orb182380
• Category: Antibodies
• Description: Cathepsin D/CTSD Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse Cathepsin D recombinant protein (Position: E65-L410). Mouse Cathepsin D shares 85% and 91% amino acid (aa) sequences identity with human and rat Cathepsin D, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Mouse Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 44954 MW
• UniProt ID: P18242
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Cathepsin D;3.4.23.5;Ctsd;
• Note: For research use only
• Application notes: WB: The detection limit for Cathepsin D is approximately 0.25ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of Cathepsin D using anti-Cathepsin D antibody.Lane 1:Mouse Liver Tissue;2:Mouse Brain Tissue;3:Mouse Thymus Tissue;4:NEURO Cell.

IHC analysis of Cathepsin D using anti-Cathepsin D antibody.Cathepsin D was detected in paraffin-embedded section of Mouse Kidney Tissue.

IHC analysis of Cathepsin D using anti-Cathepsin D antibody.Cathepsin D was detected in paraffin-embedded section of Mouse Liver Tissue.

IHC analysis of Cathepsin D using anti-Cathepsin D antibody.Cathepsin D was detected in paraffin-embedded section of rat liver tissues.