Caspase 9 antibody

• Catalog Number: orb323116
• Category: Antibodies
• Description: Mouse monoclonal antibody to Caspase 9
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence of human Caspase 9. The exact sequence is proprietary.
• Dilution range: WB: 1:1000-1:3000, IP: 1:50-1:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: CASP9
• Entrez: 842
• UniProt ID: P55211
• Source: Mouse
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti MCH6 antibody, anti Caspase-9 antibody, anti
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Caspase 9 expression in Hela (A), Jurkat (B), HepG2 (C), mouse heart (D), rat heart (E) whole cell lysates. (Predicted band size: 46 kD; Observed band size: 47 kD)

Immunofluorescent analysis of Caspase 9 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a FITC-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Immunoprecipitation of Caspase 9 from 0.5 mg Hela whole cell extract lysate, using Anti-Caspase 9 Antibody.