CASP9 Antibody

• Catalog Number: orb1239266
• Category: Antibodies
• Description: CASP9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Anti- Caspase 9 antibody (orb1239266) was raised against a peptide corresponding to 16 amino acids near the amino terminus of human Caspase 9. The immunogen is located within amino acids 30-80 of Caspase 9.
• Concentration: 1 mg/ml
• Dilution range: WB: 0.5-2 μg/mL. Antibody validated: Western Blot in human, mouse and rat samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 46kDObserved: 46kD
• Target: CASP9
• UniProt ID: P55211
• NCBI: P55211
• Storage: Caspase-9 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Caspase-9 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Caspase-9 Antibody: MCH6, APAF3, APAF-3, PPP1R56,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Independent Antibody Validation (IAV) via Protein Expression Profile in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (1 µg/mL), Caspase 9, orb1239265 (1 µg/mL) and beta-actin (1.5 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Human Heart. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 1 µg/mL, Lane 2: 2 µg/mL, Lane 3: 4 µg/mL.

Western Blot Validation in HeLa Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL, Lane 2: 1 µg/mL and Lane 3: 2 µg/mL.

Western Blot Validation in Rat Spleen Tissue. Loading: 15 µg of lysates per lane. Antibodies: Caspase 9, orb1239266 (Lane 1: 0.5 µg/mL; Lane 2: 1 µg/mL and Lane 3: 2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.