Caspase-6(P18)/CASP6 Antibody

• Catalog Number: orb27642
• Category: Antibodies
• Description: Caspase-6(P18)/CASP6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, WB
• Predicted Reactivity: Equine
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Caspase-6 (P18) (24-44aa AFYKREMFDPAEKYKMDHRRR).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 33310 MW
• UniProt ID: P55212
• Sensitivity: < 50pg/ml
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caspase-6;CASP-6;3.4.22.59;Apoptotic protease Mch-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: MCF-7 Cell Lysate, Lane 2: HELA Cell Lysate, Lane 3: JURKAT Cell Lysate, Lane 4: CEM Cell Lysate, Lane 5: SW620 Cell Lysate using Caspase 6 antibody.

Immunohistochemical analysis of formalin-fixed paraffin embedded Human Mammary Cancer Tissue using Caspase 6 antibody.