Caspase-6(P18)/CASP6 Antibody

• Catalog Number: orb137978
• Category: Antibodies
• Description: Caspase-6(P18)/CASP6 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of rat Caspase-6 (P18) (7-27aa FYRSREVLDPAEQYKMDHKRR), different from the related mouse sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, MouseWestern blot, 0.1-0.5μg/ml, Mouse, Rat
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 31556 MW
• UniProt ID: O35397
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caspase-6;CASP-6;3.4.22.59;Apoptotic protease Mch-
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Intestine Tissue using CASP6 antibody

Immunohistochemical analysis of formalin-fixed paraffin embedded Mouse Lung Tissue using CASP6 antibody

Western blot analysis of Lane 1: Rat Liver Tissue Lysate, Lane 2: Rat Kidney Tissue Lysate, Lane 3: Rat Testis Tissue Lysate, Lane 4: NRK Whole Cell Lysate, Lane 5: Mouse Liver Tissue Lysate, Lane 6: Mouse Kidney Tissue Lysate, Lane 7: Mouse Testis Tissue

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Spleen Tissue using CASP6 antibody