Caspase 4/Casp4 Antibody

• Catalog Number: orb413093
• Category: Antibodies
• Description: Caspase 4/Casp4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived mouse Caspase 4 recombinant protein (Position: E108-T195).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunocytochemistry/Immunofluorescence, 5μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 43 kDa
• UniProt ID: P70343
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caspase-4; CASP-4
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Caspase 4 using anti-Caspase 4 antibody.Lane 1:mouse liver tissue;2:mouse testis tissue;3:mouse thymus tissue;4:mouse lung tissue;5:mouse HEPA1-6 cell;6:mouse NIH3T3 cell;7:mouse SP20 cell.

WB analysis of Caspase 4 using anti-Caspase 4 antibody.Lane 1:rat liver tissue;2:rat testis tissue;3:rat stomach tissue;4:rat thymus tissue;5:human COLO-320 cell;6:human HepG2 cell;7:human 22RV1 cell;8:human PANC-1 cell;9:human SGC-7901 cell.

IF analysis of Caspase 4 using anti-Caspase 4 antibody.Caspase 4 was detected in immunocytochemical section of A431 cell.

IHC analysis of Caspase 4 using anti-Caspase 4 antibody.Caspase 4 was detected in paraffin-embedded section of human placenta tissues.