Caspase 4/CASP4 Antibody

• Catalog Number: orb402282
• Category: Antibodies
• Description: Caspase 4/CASP4 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, IHC, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Caspase 4 recombinant protein (Position: H111-R194).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Immunohistochemistry (Frozen Section), 0.5-1μg/ml Immunocytochemistry, 0.5-1μg/ml Flow Cytometry, 1-3μg/1x106 cells Immunofluorescence, 2μg/ml Direct ELISA, 0.1-0.5μg/ml
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 48 kDa
• UniProt ID: P49662
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Caspase-4; CASP-4
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of A431 cells using anti-Caspase 4 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Caspase 4 using anti-Caspase 4 antibody.Lane 1:human HeLa cell;2:human COLO-320 cell;3:human SW620 cell;4:human HepG2 cell.

IF analysis of Caspase 4 using anti-Caspase 4 antibody.Caspase 4 was detected in paraffin-embedded section of A431 cell.

IHC analysis of Caspase 4 using anti-Caspase 4 antibody.Caspase 4 was detected in paraffin-embedded section of human colon cancer tissue.

IHC analysis of Caspase 4 using anti-Caspase 4 antibody.Caspase 4 was detected in paraffin-embedded section of human mammary cancer tissue.