Caspase 3 (Cleaved-Asp175) Antibody

• Catalog Number: orb126608
• Category: Antibodies
• Description: Rabbit polyclonal antibody to caspase 3 is also known as apopain whose expression can be detected in the tonsils and skeletal muscles but is highly expressive in the spleen and kidney. It is found in the death-inducing signaling complex in the plasma membrane. This protein is extensively involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. It cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Concentration: 1mg/ml
• Immunogen: Internal
• UniProt ID: P42574
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-3000, IHC-P: 1:50-100, ELISA: 1:20000
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: anti-Apopain antibody, anti-CASP 3 antibody, anti-
• Note: For research use only

References

Zhang, Yuquan et al. Circ_0030235 knockdown protects H9c2 cells against OGD/R-induced injury via regulation of miR-526b PeerJ, 9, e11482 (2021)

PubMed: 34820154

Western blot analysis of extracts from 293 cells treated with Etoposide using Caspase 3 (Cleaved-Asp175) antibody

Immunohistochemical analysis of formalin-fixed and paraffin-embedded human lung carcinoma tissue using Caspase 3 (Cleaved-Asp175) antibody