CASP8 Antibody

• Catalog Number: orb1239264
• Category: Antibodies
• Description: CASP8 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Caspase-8 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Caspase-8 isoform A.The immunogen is located within the last 50 amino acids of Caspase-8.
• Concentration: 1 mg/mL
• Dilution range: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: CASP8
• UniProt ID: Q14790
• NCBI: NP_001219
• Storage: Caspase-8 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Caspase-8 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Caspase-8 Antibody: CAP4, MACH, MCH5, FLICE, ALPS2
• Note: For research use only
• Application notes: Caspase-8 antibody can be used for the detection of caspase-8 by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL and immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of caspase-8 in Jurkat cell lysate with caspase-8 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunohistochemistry of Caspase-8 in human spleen tissue with Caspase-8 antibody at 5 µg/mL.

Immunofluorescence of Caspase-8 in Jurkat cells with Caspase-8 antibody at 20 µg/mL.

Immunocytochemistry of caspase-8 in Jurkat cells with caspase-8 antibody at 2 µg/mL.

Western blot analysis of Caspase-8 in (A) human spleen, (B) mouse spleen, and (C) rat spleen tissue lysate with Caspase-8 antibody at 0.5 µg/ml.

Immunofluorescence of Caspase-8 in human spleen tissue with Caspase-8 antibody at 20 µg/ml. Red: Caspase-8 Antibody (orb1239264) Blue: DAPI staining.