CASP3 (Asp175) antibody

• Catalog Number: orb1430640
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CASP3 (Asp175).
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB25834
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Rat
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: WB: 1:1000, WB: 1:1000, WB: 1:1000, WB: 1:1000-1:2000, IHC-P: 1:50~100, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 31608
• Target: CASP3
• UniProt ID: P42574
• NCBI: NP_116786.1, NP_004337.2
• Alternative names: Caspase-3, CASP-3, Apopain, Cysteine protease CPP3
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CASP3 (Asp175) Antibody in CEM cell line lysates.

Western blot analysis of CASP3 (arrow) using rabbit polyclonal CASP3 Antibody.293 cell lysates either nontransfected (Lane 1) or transiently transfected (Lane 2) with the CASP3 gene.

Western blot:Anti-CASP3(Asp175) Antibody at 1:1000 dilution + Rat heart whole tissue lysate.Secondary antibody was Goat Anti-Rabbit IgG, (H+L).

Western blot:All lanes:Anti-CASP3(Asp175) Antibody at 1:1000-1:2000 dilution.Lane 1:Jurkat;Lane 2:Rat heart tissue lysate.

Immunohistochemical analysis of human brain tissue using CASP3(Asp175) Antibody, followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometric analysis of CEM cells (right histogram) using CASP3(Asp175) Antibody, compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.