CAS9 Antibody

• Catalog Number: orb1259711
• Category: Antibodies
• Description: CAS9 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Bacteria
• Isotype: IgG
• Immunogen: Recombinant protein of streptococcus pyogenes CAS9
• Concentration: batch dependent
• Dilution range: WB: 1:5000 - 1:10000IF: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 150kDa
• Target: CAS9
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: SPy_1046, SPy1046, 0
• Note: For research use only
• Application notes: WB: 1:5000 - 1:10000IF: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of wild HeLa cells and Hela transfected with cas9, using CAS9 antibody (orb1259711) at 1:5000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Immunofluorescence analysis of HeLa cells transfected with Cas9 (upper left) and untreated HeLa cells (upper right) using CAS9 Rabbit pAb (red, orb1259711) at dilution of 1:100. Blue: DAPI for nuclear staining.