Cas5 antibody

• Catalog Number: orb345557
• Category: Antibodies
• Description: Cas5 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to region near the amino terminal end of human Cas5 protein.
• Concentration: 1.3 mg/mL
• Dilution range: ELISA: 1:10,000 - 1:50,000, WB: 1:500- 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity-purified antibody is directed against human Cas5 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest cross reactivity with Cas5 from human, mouse, chimpanzee, bovine, and rat based on 100% homology with the immunizing sequence. Expect cross reactivity with multiple isoforms of this protein. Cross reactivity with Cas5 from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: B3KRV8
• NCBI: XP_016857029
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Cas5 antibody, Castor homolog 1 zinc f
• Note: For research use only
• Application notes: This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot, a band approximately 125 kDa in size corresponding to hCas5 protein is expected in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1 contains a lysate from AS cells transfected with empty vector. Lane 2 contains lysate from AS cells transfected with hCas5 (predicted MW 125 kDa). Lane 3 contains lysate from BE2 cells using Cas5 antibody