Cas 9 Antibody

SKU: orb420317

Description

Cas 9 antibody

Images & Validation

• Tested Applications: ELISA, IF, Multiplex Assay, WB
• Dilution range: ELISA: 1:25,000-1:30,000, IF: 1µg/mL, WB: 1µg/mL
• Reactivity: Bacteria
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA, Immunofluorescence, and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band ~158 kDa in size corresponding to Cas-9 protein by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Cas-9 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal of Streptococcus pyogenes Cas-9 protein.
• Purity: Anti-Cas9 antibody is directed against Streptococcus pyogenes Cas-9. The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Concentration: 1.0 mg/mL
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Cas9 antibody, Cas-9, Cas 9, CRISPR, CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9, csn1, Cas-9 Antibody

Immunofluorescence of Rabbit Anti-Cas9 Antibody. Cells: HeLa cells transfected with GFP+Cas9 or GFP+CD-3. Fixation: Formaldehyde fixed, permeabilized Triton X-100. Primary: Anti-Cas9 used at 1 µg/ml. Secondary: Donkey anti-Rabbit at 1 µg/ml, staining seen in red. Bottom images show GFP stain overlay.

Western Blot of Rabbit Anti-Cas9 Antibody. Lane 1: 293T non transfected cell lysate. Lane 2: 293T Cas9 over expressed cell lysate. Load: 15 µg per lane. Primary Antibody cas9 used at 1 µg/ml using orb348637 overnight at 4°C. Secondary Antibody: goat anti-rabbit HRP at 1:40000 for 30 min at room temp. Expect: 158kDa.

Western Blot results of Rabbit Anti-Cas 9 Antibody. Lane 1: Opal Prestained Molecular Weight Ladder. Lane 2: HEK/293 Cas9 Over Expressing WCL. Lane 3: HEK/293 non Transfected WCL. Load: 10 ul. Primary Antibody: Rabbit Anti-Cas 9 Antibody at 1 µg/ml overnight at 4°C. Secondary Antibody: Goat anti-Rabbit HRP (p/n orb347654) at 1:70000 for 30 min at RT. Blocking: BlockOut (p/n orb348644) for 30 min at RT.

Quick Database Links

UniProt

Q99ZW2

RefSeq (Protein):NP_269215.1