CARD14 Rabbit Polyclonal Antibody

SKU: orb100597

Description

CARD14 Rabbit Polyclonal Antibody

Research Area

Apoptosis, Cell Cycle

Images & Validation

• Tested Applications: ICC, WB
• Dilution range: WB=1:500-2000, ICC/IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CARD14 (1-100/1004aa)
• Target: CARD14
• Molecular Weight: 113 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Bcl10 interacting MAGUK protein 2; Bimp 2; Bimp2; CARD 14; CARD containing MAGUK 2 protein; CARD containing MAGUK protein 2; Card maguk protein 2; Carma 2; Carma2; Caspase recruitment domain containing protein 14; Caspase recruitment domain family member 14; Caspase recruitment domain protein 14; CAR14_HUMAN.

A431 cell, 4% Paraformaldehyde-fixed, Triton X-100 at room temperature for 20 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Antibody incubation with (CARD14) polyclonal Antibody, Unconjugated (orb100597) 1:100, 90 minutes at 37°C, followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

Sample: Fetal brain (Mouse) Lysate at 40 ug, Primary: Anti-CARD14 (orb100597) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113 kD, Observed band size: 113 kD.

Sample: Jurkat (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, Primary: Anti-CARD14 (orb100597) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113 kD, Observed band size: 113 kD.

Sample: Placenta (Mouse) Lysate at 40 ug, Primary: Anti-CARD14 (orb100597) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 113 kD, Observed band size: 113 kD.

Quick Database Links

Gene Symbol

CARD14

UniProt

Q9BXL6