CPA1 Antibody

SKU: orb344806

Description

Carboxypeptidase A antibody

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:250,000, WB: 1:500 - 1:3,000
• Reactivity: Bovine
• Application Notes:
  Anti-Carboxypeptidase A has been tested by western blot and is suitable for use in ELISA and immunohistochemistry. Specific conditions for reactivity should be optimized by the end user.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Carboxypeptidase A [Bovine Pancreas]
• Target: CPA1
• Purity: Anti-Carboxypeptidase A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase A [Bovine Pancreas]. Cross reactivity against Carboxypeptidase A from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

rabbit anti-Carboxypeptidase A antibody, Carboxypeptidase A1 precursor antibody, CPA1 antibody, Pancreatic carboxypeptidase A1 antibody, Procarboxypeptidase A1 pancreatic antibody

Western Blot of Rabbit Anti-Carboxypeptidase A primary antibody. Lane 1: Carboxypeptidase A. Load: 50 ng per lane. Primary antibody: Carboxypeptidase A primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody (p/n orb347654) at 1:40000 for 30 min at RT. Blocking: (p/n orb348637) for 30 min at RT. Predicted/Observed size: 34 kDa, 34 kDa for Carboxypeptidase A. Other band(s): Carboxypeptidase A splice variants and isoforms.

Quick Database Links

Gene Symbol

CPA1

UniProt

P00730

RefSeq (Protein):NP_777175.1