Carbonic Anhydrase I/CA1 Antibody

• Catalog Number: orb308824
• Category: Antibodies
• Description: Carbonic Anhydrase I/CA1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CA1 recombinant protein (Position: D9-F261). Human CA1 shares 78.5% and 81% amino acid (aa) sequence identity with mouse and rat CA1, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human Flow Cytometry, 1-3 μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 28870 MW
• UniProt ID: P00915
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Carbonic anhydrase 1;4.2.1.1;Carbonate dehydratase
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of HEL cells using anti-CA1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG. Unlabelled sample (Red line) was also used as a control.

WB analysis of CA1 using anti-CA1 antibody.Lane 1:human HEL cell; 2:rat spleen tissue; 3:mouse spleen tissue.

IF analysis of CA1 using anti-CA1 antibody. CA1 was detected in an immunocytochemical section of CACO-2 cells.

IHC analysis of CA1 using anti-CA1 antibody. CA1 was detected in a paraffin-embedded section of human adenocarcinoma of the colon cancer tissue.

IHC analysis of CA1 using anti-CA1 antibody. CA1 was detected in a paraffin-embedded section of human spleen tissue.

IHC analysis of CA1 using anti-CA1 antibody. CA1 was detected in a paraffin-embedded section of mouse colon tissue.

IHC analysis of CA1 using anti-CA1 antibody. CA1 was detected in a paraffin-embedded section of rat colon tissue.