CAPG2 Rabbit Polyclonal Antibody

• Catalog Number: orb100239
• Category: Antibodies
• Description: CAPG2 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CAPG2/NCAPG2 (451-550/1143aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 131 kDa
• Target: NCAPG2
• UniProt ID: Q86XI2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: NCAPG2; Chromosome associated protein G2; LUZP5; C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CAPG2 antibody (orb100239), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF647, Dilution: 1 µg/test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (human tonsil), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Incubation with (CAPG2) Polyclonal Antibody, Unconjugated (orb100239) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) Cell Lysate at 30 ug, Primary: Anti-CAPG2 (orb100239) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 131 kD, Observed band size: 131 kD.

Sample: Lane 1: Mouse Thymus tissue lysates, Lane 2: Rat Testis tissue lysates, Lane 3: Human A431 cell lysates, Lane 4: Human MCF-7 cell lysates, Lane 5: Human HeLa cell lysates, Lane 6: Human U251 cell lysates, Lane 7: Human Jurkat cell lysates, Primary: Anti-CAPG2 (orb100239) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 131 kDa, Observed band size: 131 kDa.