Canine NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit

• Catalog Number: orb1946497
• Category: Assays and Kits
• Description: Canine NT-ProBNP(N-Terminal Pro-Brain Natriuretic Peptide) ELISA Kit
• Reactivity: Canine
• Range: 78.13-5000 pg/mL
• Target: NT-ProBNP
• Sensitivity: 24.2 pg/mL
• Alternative names: NT-Pro-BNP; ; N-BNP; ANFB_HUMAN; BNP(1-32); BNP(5-
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of N-Terminal Pro-Brain Natriuretic Peptide(NT-ProBNP) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.