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Catalog Number | orb429416 |
---|---|
Category | Proteins |
Description | Recombinant of canine IL8 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-8 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | AVLSRVSSEL RCQCIKTHST PFHPKYIKEL RVIDSGPHCE NSEIIVKLFN GNEVCLDPKE KWVQKVVQIF LKKAEKQDP |
Source | Escherichia Coli |
Biological Activity | The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.15-0.75 ng/ml. |
Storage | Stability: Lyophilized IL-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL8 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4. |
Alternative names | Interleukin-8, IL-8, C-X-C motif chemokine 8, IL8, Read more... |
Note | For research use only |
Application notes | Chemokines |
Expiration Date | 6 months from date of receipt. |
> 95 % by SDS-PAGE and HPLC analyses. | |
Approximately 9.1 kDa, a single non-glycosylated polypeptide chain containing 79 amino acids. | |
Escherichia coli |
SDS-PAGE | |
Unconjugated | |
> 95% by SDS-PAGE and HPLC analyses. | |
9.1 kDa | |
E. coli |
Greater than 90.0% as determined by SDS-PAGE. | |
HEK293 Cells |