Canine I-PTH(IntactParathormone) ELISA Kit

• Catalog Number: orb1946485
• Category: Assays and Kits
• Description: Canine I-PTH(IntactParathormone) ELISA Kit
• Reactivity: Canine
• Range: 12.5-800 pg/mL
• Target: I-PTH
• Sensitivity: 4.15 pg/mL
• Alternative names: iPTH; Intact Parathyroid Hormone; Parathormone; Pa
• Note: For research use only
• Application notes: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IntactParathormone(I-PTH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IntactParathormone(I-PTH). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IntactParathormone(I-PTH) in the samples is then determined by comparing the OD of the samples to the standard curve.
• Sample Types: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.