CaMK2 alpha Rabbit Polyclonal Antibody

• Catalog Number: orb156259
• Category: Antibodies
• Description: CaMK2 alpha Rabbit Polyclonal Antibody
• Target: CAMK2A
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human CaMK2 alpha (101-200/478aa)
• UniProt ID: Q9UQM7
• MW: 54 kDa
• Tested applications: ICC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: alpha CaMKII; CaMKII alpha; Calcium/Calmodulin Dep
• Research Area: Cardiovascular Research, Hypoxia, Signaling Transd
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug total protein per lane of various lysates probed with CaMK2 alpha polyclonal antibody, unconjugated (orb156259) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

4% Paraformaldehyde-fixed SH-SY5Y (H) cell, Triton X-100 at r.t. for 20 min, Antibody incubation with (CaMK2 alpha) polyclonal Antibody, unconjugated (orb156259) 1:100, 90 min at 37°C, followed by conjugated Goat Anti-Rabbit IgG antibody (green) at 37°C for 90 min, DAPI (blue) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CaMK2 alpha) Polyclonal Antibody, Unconjugated (orb156259) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Mouse Heart tissue lysates, Lane 2: Rat Heart tissue lysates, Primary: Anti-CaMK2 alpha (orb156259) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kDa, Observed band size: 50 kDa.