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Calreticulin Rabbit Polyclonal Antibody (FITC)

Catalog Number: orb102221

DispatchUsually dispatched within 2-3 weeks
$ 450.00
Catalog Numberorb102221
CategoryAntibodies
DescriptionCalreticulin Rabbit Polyclonal Antibody (FITC)
TargetCALR
ClonalityPolyclonal
Species/HostRabbit
IsotypeIgG
ConjugationFITC
ReactivityHuman
Predicted ReactivityBovine, Canine, Equine, Gallus, Guinea pig, Porcine, Rabbit
Concentration1mg/ml
Buffer/Preservatives0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
ImmunogenKLH conjugated synthetic peptide derived from human Calreticulin (101-200/417aa)
Tested applicationsIF
Dilution rangeIF=1:100-500
Antibody TypePrimary Antibody
StorageShipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Alternative namesAutoantigen RO; CALR; CALR protein; CALR_HUMAN; cC
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NoteFor research use only
Calreticulin Rabbit Polyclonal Antibody (FITC)

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Calreticulin antibody (orb102221), Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

Calreticulin Rabbit Polyclonal Antibody (FITC)

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Calreticulin antibody, Dilution: 1 ug/Test, Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.

  • Calreticulin Antibody: FITC [orb151514]

    ICC,  IF,  IHC

    Bovine, Canine, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep

    Rabbit

    Polyclonal

    FITC

    200 μl