Calreticulin Antibody

SKU: orb1822435

Description

Rabbit Anti-Human Calreticulin Polyclonal

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, IP, WB
• Dilution range: WB (1:1000), IHC (1:100), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
• Reactivity: Bovine, Canine, Gallus, Guinea pig, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
• Application Notes:
  A 1:1000 dilution of SPC-122 was sufficient for detection of Calreticulin in 20 µg of HeLa cell lysate by ECL immunoblot analysis.

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Human calreticulin synthetic peptide with a cysteine residue added and the peptide conjugated to KLH
• Target: Calreticulin
• Molecular Weight: 63kDa
• Purification: Peptide Affinity Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS pH 7.4, 50% glycerol, 0.09% sodium azide *Storage buffer changes when conjugated
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

Calreticulin, CALR, CALR_HUMAN, Calregulin, cC1qR, CRP55, ERp60, HSCBP, RO, SSA, grp60

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Western blot analysis of multiple cell lines lysates showing detection of Calreticulin protein using Rabbit Anti-Calreticulin Polyclonal Antibody. Load: 15 μgprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody at 1:5000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.

Immunohistochemistry analysis using Rabbit Anti-Calreticulin Polyclonal Antibody. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative Solution. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic granule. Endoplasmic reticulum lumen.

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Calreticulin Polyclonal Antibody. Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Calreticulin Polyclonal Antibody at 1:100 for 12 hours at 4°C. Secondary Antibody: R-PE Goat Anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x. (A) DAPI (blue) nuclear stain. (B) Anti-Calreticulin Antibody. (C) Composite. Heat Shocked at 42°C for 1h.

Quick Database Links

Gene Symbol

Calreticulin

UniProt

P27797

NCBI Gene ID

811

RefSeq (Protein):NP_004334.1