CALR Antibody

• Catalog Number: orb1788282
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Target: This CALR Antibody is generated from rabbits immunized with a recombinant protein of human CALR.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Rat
• Predicted Reactivity: Mouse
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Immunogen: 1-437 aa
• UniProt ID: P27797
• MW: 48142 Da
• Tested applications: FC, IHC-P, WB
• Dilution range: WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: CALR; CRTC; Calreticulin; CRP55; Calregulin; Endop
• Note: For research use only
• NCBI: NP_004334.1

CALR Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of lysates from rat C6, Hela, SH-SY5Y cell line (from left to right), using CALR Antibody. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

CALR Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CALR Antibody for immunohistochemistry. Clinical relevance has not been evaluated.