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Catalog Number | orb1263782 |
---|---|
Category | Antibodies |
Description | Calponin-3 Antibody |
Target | CNN3 |
Clonality | Polyclonal |
Isotype | Rabbit Ig |
Conjugation | Unconjugated |
Reactivity | Human |
Predicted Reactivity | Bovine, Mouse, Rat |
Form/Appearance | Liquid |
Concentration | batch dependent |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Purification | This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis |
Immunogen | This Calponin-3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the Central region of human Calponin-3. |
UniProt ID | Q15417 |
MW | 36 kDa |
Tested applications | FC, IHC-P, WB |
Application notes | For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50 |
Antibody Type | Primary Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | Calponin-3, Calponin, acidic isoform, CNN3 |
Note | For research use only |
NCBI | Q15417 |
Western blot analysis of Calponin-3 antibody in NIH-3T3 cell line lysates (35 ug/lane)
Calponin-3 Antibody IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of NIH-3T3 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ELISA, IHC, WB | |
Human | |
Goat | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Mouse, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |