Calponin 1 + 2 + 3 Rabbit Polyclonal Antibody

• Catalog Number: orb2780
• Category: Antibodies
• Description: Calponin 1 + 2 + 3 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P
• Predicted Reactivity: Bovine, Canine, Equine, Human, Mouse, Porcine
• Reactivity: Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Calponin 1/2/3 (101-200/309aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:50-200, Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 34 kDa
• Target: CNN2
• UniProt ID: Q99439
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: smooth muscle; Basic calponin; Calponin 1; Calponi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Rat heart), Antigen retrieval by microwave in sodium citrate buffer (pH6.0), Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (3% BSA) at RT for 30 min, Antibody incubation with (Calponin 1 + 2 + 3) Polyclonal Antibody, Unconjugated (orb2780) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP) and DAB staining.

Positive control: H9C2 (2% Paraformaldehyde-fixed), Isotype Control Antibody: Rabbit IgG, dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 1:200 in 1X PBS containing 0.5% BSA, Primary Antibody: rabbit Anti-Calponin 1 + 2 + 3 (orb2780), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA.