Calpain 2/CAPN2 Antibody

• Catalog Number: orb402529
• Category: Antibodies
• Description: Calpain 2/CAPN2 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human Calpain 2 recombinant protein (Position: R500-L700). Human Calpain 2 shares 93.5% and 92.5% amino acid (aa) sequence identity with mouse and rat Calpain 2, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat, Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 79995 MW
• UniProt ID: P17655
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calpain-2 catalytic subunit;3.4.22.53;Calcium-acti
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U87 cells using anti-Calpain 2 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Calpain 2 using anti-Calpain 2 antibody.Lane 1:human HepG2 cell; 2:human A431 cell; 3:human HeLa cell; 4:rat kidney tissue; 5:rat brain tissue; 6:mouse kidney tissue; 7:mouse brain tissue.

IF analysis of Calpain 2 using anti-Calpain 2 antibody. Calpain 2 was detected in immunocytochemical section of HELA cells.

IHC analysis of Calpain 2 using anti-Calpain 2 antibody.Calpain 2 was detected in paraffin-embedded section of rat small intestine tissue.

IHC analysis of Calpain 2 using anti-Calpain 2 antibody.Calpain 2 was detected in paraffin-embedded section of human intestinal cancer tissue.

IHC analysis of Calpain 2 using anti-Calpain 2 antibody.Calpain 2 was detected in paraffin-embedded section of human lung cancer tissue.