Calpain 1/CAPN1 Antibody

• Catalog Number: orb27577
• Category: Antibodies
• Description: Calpain 1/CAPN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, IHC-Fr, WB
• Predicted Reactivity: Equine
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Calpain 1 (312-326aa EWNNVDPYERDQLRV), different from the mouse sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat, Human, Mouse Immunocytochemistry , 0.5-1μg/ml, Human, - Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81890 MW
• UniProt ID: P07384
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calpain-1 catalytic subunit;3.4.22.52;Calcium-acti
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Lane 1: HT1080 Cell Lysate, Lane 2: COLO320 Cell Lysate using Calpain 1 antibody.

Immunohistochemical analysis of formalin-fixed paraffin embedded Rat Lung Tissue using Calpain 1 antibody.

Immunohistochemical analysis of formalin-fixed paraffin embedded HELA Cell using Calpain 1 antibody.