Calpain 1/CAPN1 Antibody

• Catalog Number: orb251513
• Category: Antibodies
• Description: Calpain 1/CAPN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human Calpain 1 recombinant protein (Position: Q396-A555). Human Calpain 1 shares 86% amino acid (aa) sequence identity with both mouse and rat Calpain 1.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 81890 MW
• UniProt ID: P07384
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calpain-1 catalytic subunit;3.4.22.52;Calcium-acti
• Note: For research use only
• Application notes: WB: The detection limit for Calpain 1 is approximately 0.1ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of PC-3 cells using anti-Calpain 1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of Calpain 1 using anti-Calpain 1 antibody.Lane 1:human Jurkat cell; 2:human HeLa cell; 3:human K562 cell; 4:human PC-3 cell; 5:human T47D cell; 6:human A549 cell; 7:rat testis tissue; 8:mouse lung tissue.

IF analysis of Calpain 1 using anti-Calpain 1 antibody. Calpain 1 was detected in immunocytochemical section of U20S cells.

IHC analysis of Calpain 1 using anti-Calpain 1 antibody. Calpain 1 was detected in a paraffin-embedded section of Human Mammary Cancer tissue.