Calnexin Rabbit Polyclonal Antibody

• Catalog Number: orb10428
• Category: Antibodies
• Description: Calnexin Rabbit Polyclonal Antibody
• Target: CANX
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Bovine, Equine, Gallus, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human CANX (501-592/592aa)
• UniProt ID: P27824
• RRID: AB_10746430
• MW: 65 kDa
• Tested applications: FC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1μg /test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Calnexin-ER membrane marker; CANX; Cell adhesion m
• Note: For research use only

Blank control: H9C2 Cells (blue). Primary Antibody: Rabbit Anti-CANX/FITC Conjugated antibody, Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG/FITC (orange), used under the same conditions. Protocol, The cells were washed twice with phosphate-buffered saline (PBS). The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the antibody (1 µg/1x10^6 cells) for 30 min on ice. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Human K562 cell lysates, Lane 6: Human HeLa cell lysates, Lane 7: Human HepG2 cell lysates, Lane 8: Human U-2 OS cell lysates, Lane 9: Human MCF-7 cell lysates, Lane 10: Human A431 cell lysates, Primary: Anti-Calnexin (orb10428) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 65 kDa, Observed band size: 78 kDa.