Calmodulin antibody

• Catalog Number: orb764704
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Calmodulin
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Calmodulin. AA range:46-95
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Store at -20°C. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-CALM1 antibody, anti-CALM antibody, anti-CAM
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of Hela cell. 1, Calmodulin Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). (red) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min).

Western blot analysis of lysates from 1) HuvEc, 2) HepG2, 3) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) Actin β Monoclonal Antibody (5B7) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.

Western Blot analysis of various cells using Calmodulin Polyclonal Antibody diluted at 1:2000.

Western Blot analysis of HuvEc cells using Calmodulin Polyclonal Antibody diluted at 1:2000.