CaMKII alpha/CAMK2A Antibody

• Catalog Number: orb654314
• Category: Antibodies
• Description: CaMKII alpha/CAMK2A Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, ICC, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CaMKII alpha/CAMK2A recombinant protein (Position: M1-H478).
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.25-0.5μg/ml, Mouse, Rat Immunocytochemistry/Immunofluorescence, 5μg/ml, Human Immunofluorescence, 5μg/ml, Rat Flow Cytometry, 1-3μg/1x106 cells, Human, Mouse, Rat Direct ELISA, 0.1-0.5μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 54 kDa
• UniProt ID: Q9UQM7
• Sensitivity: > 5000 cells
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Calcium/calmodulin-dependent protein kinase type I
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CaMKII alpha/CAMK2A using anti-CaMKII alpha/CAMK2A antibody (orb654314). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat brain tissue lysates, Lane 3: mouse brain tissue lysates, Lane 4: mouse brain tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII alpha/CAMK2A antigen affinity purified polyclonal antibody (Catalog # orb654314) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CaMKII alpha/CAMK2A at approximately 54KD. The expected band size for CaMKII alpha/CAMK2A is at 54KD.

Flow Cytometry analysis of U87 cells using anti-CaMKII alpha/CAMK2A antibody (orb654314). Overlay histogram showing U87 cells stained with orb654314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII alpha/CAMK2A Antibody (orb654314, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of ANA-1 cells using anti-CaMKII alpha/CAMK2A antibody (orb654314). Overlay histogram showing ANA-1 cells stained with orb654314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII alpha/CAMK2A Antibody (orb654314, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Flow Cytometry analysis of NRK cells using anti-CaMKII alpha/CAMK2A antibody (orb654314). Overlay histogram showing NRK cells stained with orb654314 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CaMKII alpha/CAMK2A Antibody (orb654314, 1μg/1x10^6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x10^6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

IF analysis of CaMKII alpha/CAMK2A using anti-CaMKII alpha/CAMK2A antibody (orb654314). CaMKII alpha/CAMK2A was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (orb90553) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti-CaMKII alpha/CAMK2A Antibody (orb654314) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

IF analysis of CaMKII alpha/CAMK2A using anti-CaMKII alpha/CAMK2A antibody (orb654314). CaMKII alpha/CAMK2A was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-CaMKII alpha/CAMK2A Antibody (orb654314) overnight at 4°C. Biotin conjugated goat anti-rabbit IgG (orb1474935) was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using DyLight®488 Conjugated Avidin. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.