Calcitonin Rabbit Polyclonal Antibody

• Catalog Number: orb500533
• Category: Antibodies
• Description: Calcitonin Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Canine, Guinea pig, Human, Rabbit, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Calcitonin (85-116/141aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 3.5/13 kDa
• Target: CALCA
• UniProt ID: P01258
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: alpha CGRP; CALC 1; CALC A; CALC1; CALCA; Calciton
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calcitonin) Polyclonal Antibody, Unconjugated (orb500533) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Calcitonin (orb500533) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 3.5/13 kD, Observed band size: 17 kD.

Sample: Lane 1: Mouse Spinal cord tissue lysates, Lane 2: Mouse Cerebrum tissue lysates, Lane 3: Rat Spinal cord tissue lysates, Lane 4: Rat Cerebrum tissue lysates, Lane 5: Human TT cell lysates, Primary: Anti-Calcitonin (orb500533) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 3.5/13 kDa, Observed band size: 17 kDa.