Calcineurin B Rabbit Polyclonal Antibody

SKU: orb156248

Description

Calcineurin B Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine, Rabbit, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Calcineurin B/PP2B-B1 (31-130/170aa)
• Target: PPP3R1
• Molecular Weight: 19 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Calcineurin subunit B type 1; PP2B-B1; CALNB1; CANB1_HUMAN; Cna2; CNB; CNB1; OTTHUMP00000201960; OTTHUMP00000201961; Ppp3r1; PPP3R1 protein phosphatase 3(formerly 2B), regulatory subunit B, alpha isoform; alpha isoform(calcineurin B, type I); calcineurin B, type I(19kDa); protein phosphatase3(formerly2B), regulatory subunit B, alpha isoform antibody Protein phosphatase 2B regulatory subunit 1; Protein phosphatase 2B regulatory subunit B alpha; protein phosphatase 3(formerly 2B), regulatory subunit B, 19kDa, alpha isoform(calcineurin B, type I); Protein phosphatase 3 regulatory subunit B alpha; Protein phosphatase 3 regulatory subunit B alpha isoform 1.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Calcineurin B) Polyclonal Antibody, Unconjugated (orb156248) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: HL-60 (Human) cell Lysate at 40 ug, Primary: Anti-Calcineurin B (orb156248) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 19kD, Observed band size: 19kD.

Quick Database Links

Gene Symbol

PPP3R1

UniProt

P63098