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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-127 of human CALCB (NP_000719.1). |
| Target | CALCB |
| Molecular Weight | Observed: 14kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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ELISA, ICC, IF, IHC
Human
Rabbit
Polyclonal
Unconjugated
200 μl, 100 μl, 50 μl

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Western blot analysis of extracts of various cell lines, using CALCB antibody (orb1255994) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Immunohistochemistry of paraffin-embedded rat heart using CALCB antibody (orb1255994) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human prostate using CALCB antibody (orb1255994) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using CALCB antibody (orb1255994) at dilution of 1:100 (40x lens).
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Protocol Information
CALCB Antibody (orb1255994)
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