CALB1 Protein

• Catalog Number: orb424478
• Category: Proteins
• Description: Recombinant of CALB1 protein
• Form/Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Buffer/Preservatives: The protein was lyophilized from a concentrated solution (1mg/ml) containing 50mM NaHCO3.
• Purity: Greater than 90% as determined by SDS-PAGE.
• Application notes: Applications: CABP28K can be used for immunoblots, absorption experiments in immunohistochemistry, radioimmunoassay and intracellular injection.For adsorption we suggest the following procedureA- Dilute 1 µl of the monoclonal antibody against calbindin D-28k in 5 ml of the usual buffer for immunohistochemistry (final dilution 1:5'000).B- Add 1 µg of the recombinant protein to 1 ml of the diluted antibody solution and mix well.C- Incubate for at least 6 hours in the cold.D- Apply to tissue-sections and incubate for 3 days.E- Complete the immunohistochemical reaction as usual (biotinylated second antibody, ABC-complex, DAB).As a result, the immunostaining should be strongly reduced or even completely prevented
• Source: Escherichia Coli
• Solubility (25°C): It is recommended to reconstitute the lyophilized CABP28K in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Stability: Lyophilized CABP28K although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CABP28K should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
• Alternative names: Calbindin, Vitamin D-dependent calcium-binding pro
• Note: For research use only