C6ORF199 Rabbit Polyclonal Antibody

SKU: orb156111

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Description

Images & Validation

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Tested Applications	IF, IHC-Fr, IHC-P, WB
Dilution range	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
Reactivity	Human, Rat
Predicted Reactivity	Mouse

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	KLH conjugated synthetic peptide derived from human C6ORF199 (1601-1700/1911aa)
Target	AK9
Molecular Weight	221 kDa
Purification	Affinity purified by Protein A
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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adenylate kinase domain containing 1; adenylate kinase domain containing 2; Adenylate kinase domain-containing protein 1; Adenylate kinase domain-containing protein 2; AKD1; AKD1_HUMAN; AKD2; C6orf224; chromosome 6 open reading frame 199; chromosome 6 open reading frame 224; dJ70A9.1; FLJ16163; FLJ25791; FLJ34784; FLJ42177; Gm234; Gm7127; MGC126763; MGC138153; MGC177059; MGC180194; MGC184281; MGC26954; OTTHUMP00000040459; OTTHUMP00000040461; RP1-70A9.

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Paraformaldehyde-fixed, paraffin embedded (Rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (C6ORF199) Polyclonal Antibody, Unconjugated (orb156111) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructions and DAB staining.

Sample: HepG2 (Human) cell Lysate at 40 ug, Primary: Anti-C6ORF199 (orb156111) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 221kD, Observed band size: 245kD.

Quick Database Links

Gene Symbol

AK9

UniProt

Q5TCS8

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC-P

Immunohistochemistry Paraffin

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IHC-Fr

Immunohistochemistry Frozen

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Immunofluorescence

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