C4A & C4B Antibody

• Catalog Number: orb1252116
• Category: Antibodies
• Description: C4A & C4B Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CPT4d-1
• Tested applications: IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Human protein was used as the immunogen for this C4d antibody.
• Concentration: 0.2 mg/mL
• Dilution range: IF: 1:50-1:100IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: C4A & C4B
• UniProt ID: P0C0L4, P0C0L5
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Note: For research use only
• Application notes: IF: 1:50-1:100IHC (FFPE): 1:200-1:400 for 30 minutes at RT (1)The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the C4d antibody to be titered up or down for optimal performance.1. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human kidney transplant tissue stained with C4d antibody (CPT4d-1).