C4A, C4B Antibody

• Catalog Number: orb1251876
• Category: Antibodies
• Description: C4A, C4B Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: SPM545
• Tested applications: ELISA, IF, IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Immunogen: Recombinant human C4d protein was used as the immunogen for this Complement 4d antibody.
• Concentration: 0.2 mg/mL
• Dilution range: ELISA: 1-2 ug/ml (order BSA/azide free format)Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Complement 4d antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: C4A, C4B
• UniProt ID: P0C0L4, P0C0L5
• Storage: Aliquot and Store at 2-8°C. Avoid freez-thaw cycles.
• Buffer/Preservatives: PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Alternative names: Complement C4-A, Acidic complement C4, C3 and PZP-
• Note: For research use only
• Application notes: ELISA: 1-2 ug/ml (order BSA/azide free format)Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 minutes at RT (1)Prediluted format: incubate for 30 min at RT (2)The optimal dilution of the Complement 4d antibody for each application should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Formalin-fixed, paraffin-embedded human kidney transplant stained with Complement 4d antibody (SPM545).